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ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L) (100 μl)
| Applications: | IF/IC, FC |
| Host Species: | Goat |
| Isotype: | IgG |
| Conjugation: | ABflo® 750. Ex:750nm. Em:775nm. |
| Immunogen: | Rabbit IgG |
| Dilution: | IF/IC 1:500-1:1000; FC 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/mL |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Confocal imaging of paraffin-embedded Mouse brain using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with ABflo® 750-conjugated Goat Anti-Rabbit IgG (H+L)(AS072, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining. |
 | Confocal imaging of paraffin-embedded Mouse brain using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with ABflo® 750-conjugated Goat Anti-Rabbit IgG (H+L)(AS072, dilution 1:1000) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining. |
 | Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2.5μg/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 750-conjugated Goat Anti-Rabbit IgG (H+L) (AS072) at 1:100. |
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