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FITC-conjugated Goat anti-Mouse IgG (H+L) (100 μl)
| Applications: | IF/IC, FC |
| Host Species: | Goat |
| Isotype: | Fluorescein conjugated IgG |
| Conjugation: | FITC. Ex:491nm. Em:516nm. |
| Immunogen: | Mouse IgG |
| Dilution: | IF/IC 1:100-1:500; FC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/mL |
| Buffer: | PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by a further incubation with FITC Goat Anti-Mouse IgG (H+L) (AS001, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometric analysis of Positive antibody (AE005) (2.5μg/mL) in various cells (orange) compare to Mouse isotype control (blue) and non-staining control (Red).The secondary antibody used was FITC Goat Anti-Mouse IgG (H+L) (AS001) at 1:100. |
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