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Applications: | WB, ELISA |
Host Species: | Goat |
Isotype: | IgG |
Conjugation: | Unconjugated |
Immunogen: | Rabbit IgG |
Dilution: | WB 1:1000-1:6000; CUT&Tag, 1:100 |
Purification Method: | Affinity purification |
Concentration: | 1.08 mg/mL |
Buffer: | PBS with 0.05% proclin300,50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual |
Images
![]() | CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K-562 cells with 1 μg of TriMethyl-Histone H3-K4 Rabbit mAb (A22146), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of TriMethyl-Histone H3-K4 around RPL30 gene. |
![]() | CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K-562 cells with 1 μg of TriMethyl-Histone H3-K4 Rabbit mAb (A22146), followed by incubation with Goat Anti-Rabbit IgG (H+L)(AS070). The CUT&Tag results denote the enrichment pattern of TriMethyl-Histone H3-K4 across chromosome 8 (upper panel) and the genomic region encompassing RPL30, a representative gene enriched in TriMethyl-Histone H3-K4 (lower panel). |
![]() | Western blot analysis of recombinant Rabbit/Mouse/Rat control IgG Protein using Goat Anti-Rabbit IgG(H+L) (AS070) at 1:1000 dilution. Secondary antibody: HRP Donkey Anti-Goat IgG (H+L) (AS031) at 1:10000 dilution. Lysates/proteins: 100ng/50ng/25ng/10ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |