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HRP-conjugated Donkey anti-Mouse IgG (H+L) (100 μl)
| Applications: | WB, IHC |
| Host Species: | Donkey |
| Isotype: | Horseradish peroxidase conjugated IgG |
| Conjugation: | HRP |
| Immunogen: | Mouse IgG |
| Dilution: | WB 1:10000-1:30000; IHC 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.4 mg/mL |
| Buffer: | PBS with 0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of recombinant Mouse control IgG Protein using HRP Donkey Anti-Mouse IgG (H+L)(AS033) at 1:10000 dilution. Secondary antibody:HRP Donkey Anti-Mouse IgG (H+L)(AS033) at 1:10000 dilution. Lysates/proteins: 100ng/50ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Western blot analysis of lysates from HeLa cells, using GAPDH antibody as the primary antibody. Secondary antibody:HRP Donkey Anti-Mouse IgG (H+L)(AS033) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain using HRP Donkey Anti-Mouse IgG (H+L) (AS033) at dilution of 1:20 - 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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