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HRP-conjugated Donkey anti-Rabbit IgG (H+L) (200 μl)
| Applications: | WB, IHC |
| Host Species: | Donkey |
| Isotype: | Horseradish peroxidase conjugated IgG |
| Conjugation: | HRP |
| Immunogen: | Rabbit IgG |
| Dilution: | WB 1:5000-1:100000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.4 mg/mL |
| Buffer: | PBS with 0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of lysates from HeLa cells, using β-actin antibody as the primary antibody. Secondary antibody: HRP Donkey Anti-Rabbit IgG (H+L) antibody (AS038) at 1:2000-1:30000 dilution Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver (primary antibody is acox1) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver (the primary antibody is acox1, And the control secondary antibody is stained) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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