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HRP-conjugated Goat anti-Rabbit IgG (H+L) (100 μl)
| Applications: | WB, DB, IHC, ELISA |
| Host Species: | Goat |
| Isotype: | Horseradish peroxidase conjugated IgG |
| Conjugation: | HRP |
| Immunogen: | Rabbit IgG |
| Dilution: | WB 1:2000-1:10000; DB 1:2000-1:10000; IHC 1:50-1:200; ELISA 1:5000-1:10000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.13 mg/mL |
| Buffer: | PBS with 0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000. Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry analysis of paraffin-embedded Human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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