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HRP-conjugated Rabbit anti-Human IgM mAb (100 μl)
| Applications: | ELISA |
| Host Species: | Rabbit |
| Isotype: | |
| Conjugation: | HRP |
| Clone ID: | 9V7D2 |
| Dilution: | ELISA 1:5000-1:10000 |
| Concentration: | 0.65 mg/mL |
| Buffer: | PBS with 0.05% proclin300,0.05% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Dose response curve of HRP conjugated Human IgM mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgM mAb in a 3 fold serial dilution from 0.5 μg/mL to 3.05×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Human IgM mAb has highly specific recognition of Human IgM while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Mouse IgM、Rat IgM. |
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