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TRITC-conjugated Goat anti-Rabbit IgG (H+L) (200 μl)
| Applications: | IHC, IF/IC, FC |
| Host Species: | Goat |
| Isotype: | TRITC conjugated IgG |
| Conjugation: | Rhodamine. Ex:550nm. Em:570nm. |
| Immunogen: | Rabbit IgG |
| Dilution: | IHC 1:50-1:200; IF/IC 1:50-1:200; FC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/mL |
| Buffer: | PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Immunofluorescence analysis of HeLa cells, using Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:200 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells, using Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (AS040) at 1:100 dilution. Blue: DAPI for nuclear staining. |
 | Flow cytometry: 1X10^6 K-562 cells (negative control,left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test,orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)(AS040, 1:200) was used as a secondary antibody. |
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