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| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Gene Name: | tubulin alpha 4a |
| Gene Symbol: | TUBA4A |
| Synonyms: | ALS22; TUBA1; H2-ALPHA; α-Tubulin |
| Gene ID: | 7277 |
| UniProt ID: | P68366 |
| Clone ID: | 11B4E1 |
| Immunogen: | Recombinant protein of human α-Tubulin |
| Dilution: | WB 1:5000-1:10000; IHC 1:50-1:200; IF/IC 1:100-1:200; IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of various lysates using α-Tubulin Mouse mAb (AC012) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain using α-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using α-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:200)(Green). The cells were counterstained with USO1 Rabbit mAb (A20950, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x. |
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