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| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Gene Name: | tubulin alpha 1b |
| Gene Symbol: | TUBA1B |
| Synonyms: | K-ALPHA-1; α-Tubulin |
| Gene ID: | 10376 |
| UniProt ID: | P68363 |
| Clone ID: | 2L5F2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-42 of human α-Tubulin(NP_006073.2). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of lysates from HeLa cells, using α-Tubulin Rabbit mAb (AC049) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5s. |
 | Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using α-Tubulin Rabbit mAb (AC049) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using α-Tubulin Rabbit mAb (AC049) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using α-Tubulin Rabbit mAb (AC049) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
| Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using α-Tubulin Rabbit mAb (AC049) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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