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| Applications: | WB, IF/IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Gene Name: | tubulin alpha 4a |
| Gene Symbol: | TUBA4A |
| Synonyms: | ALS22; TUBA1; H2-ALPHA; α-Tubulin |
| Gene ID: | 7277 |
| UniProt ID: | P68366 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human α-Tubulin (NP_005991.1). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 3.48 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of various lysates using α-Tubulin Rabbit pAb (AC007) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of various lysates, using α-Tubulin Rabbit pAb (AC007) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Confocal immunofluorescence analysis of U2OS cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using α-Tubulin Rabbit pAb (AC007) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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