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| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Gene Name: | actin beta |
| Gene Symbol: | ACTB |
| Synonyms: | BRWS1; PS1TP5BP1; β-Actin |
| Gene ID: | 60 |
| UniProt ID: | P60709 |
| Clone ID: | 3I3T4 |
| Immunogen: | Recombinant protein of human β-Actin |
| Dilution: | WB 1:10000-1:140000; IHC 1:500-1:1000; IF/IC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of various lysates, using β-Actin Rabbit mAb (AC048) at 1:120000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using β-Actin Rabbit mAb (AC048) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse testis using β-Actin Rabbit mAb (AC048) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Confocal imaging of U-2 OS cells using β-Actin Rabbit mAb (AC048, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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