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| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Gene Name: | tubulin beta class I |
| Gene Symbol: | TUBB |
| Synonyms: | M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56; β-Tubulin |
| Gene ID: | 203068 |
| UniProt ID: | P07437 |
| Clone ID: | 4E9G8 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human β-Tubulin (P07437). |
| Dilution: | WB 1:5000-1:10000; IHC 1:50-1:200; IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Purification Method: | Affinity purification |
| Concentration: | 0.75 mg/ml |
| Buffer: | PBS with 0.02% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of various lysates using β-Tubulin Rabbit mAb (A12289) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Western blot analysis of lysates from 293F cells, using β-Tubulin Rabbit mAb (A12289) at 1:1000-1:30000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using β-Tubulin Rabbit mAb (A12289) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of C6 cells using β-Tubulin Rabbit mAb (A12289) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using β-Tubulin Rabbit mAb (A12289) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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