Anti-BMP2 Rabbit pAb (100 μl)

Anti-BMP2 Rabbit pAb (100 μl)

Brand: ServiceBio
Cat. #: GB11252-100
Availability: In Stock
$259.00
-+
Reactivity: M,R    
Applications: WB    
Host Species: Rabbit    
Clonality: Polyclonal    
Full Name:

BMP2 rabbit polyclonal antibody

Gene Name:

Bone morphogenetic protein 2

Synonyms:

BMP2, BDA2, BMP2A, bone morphogenetic protein 2

Immunogen:

KLH conjugated Synthetic peptide corresponding to Mouse BMP2

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Secreted

Predicted MW.

45 kDa

Observed MW.

45 kDa

Uniprot ID:

P12643, P21274, P49001




Product Usage Information

WB

Human, Mouse, Rat

1: 500-1: 1000

liver, spleen, lung

IHC/IF

Human (predicted), Mouse, Rat

1: 200-1: 1000

bone




Background

Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.




Images


WB analysis of BMP2 (GB11252).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

IHC analysis of BMP2 (GB11252).
Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.


IHC analysis of BMP2 (GB11252).
Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.




Storage

Storage Store at -20°C for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.


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