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| Reactivity: | H & M & H |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Transcription factor IIIB 50 kDa subunit |
Synonyms: | B related factor 2, BRF 2, BRF2, BRFU, hBRFU, hTFIIIB50, TFIIIB50 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse BRF2 |
Uniprot ID: | Q3UAW9 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 47 kDa / 47 kDa |
Product Usage Information
WB | Human | 1: 800-1: 1500 | HeLa, A549, HCT 116 |
IHC/IF | Mouse | 1: 800-1: 2400 | pancreas, colon |
ICC/IF | Human | 1: 100-1: 400 | HeLa |
Background
General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.
Images
| Western blot analysis of BRF2 (GB114896) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded mouse pancreas using BRF2 (GB114896) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded mouse colon using BRF2 (GB114896) at dilution of 1: 800 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using BRF2 (GB114896) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
