Recombinant Anti-Bax Rabbit mAb (100 μl)

Recombinant Anti-Bax Rabbit mAb (100 μl)

Brand: ServiceBio
Cat. #: GB154122-100
Availability: In Stock
$259.00
-+
Reactivity: Human, Mouse, Rat    
Applications: IHC    
Host Species: Rabbit    
Clonality: Monoclonal antibody    
Gene Name: BCL2-associated X protein
Gene Symbol: Bax
Synonyms: BAX, BCL2L4, BCL2 associated X protein, BCL2 associated X, Apoptosis regulator BAX, Bcl-2-Like Protein 4,Bcl2-L-4, Bax-protein
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse Bax
Gene ID: 12028
Uniprot ID: Q07812
Isotype: IgG
Purity: Affinity purification
Subcellular location: Mitochondrion, Cytoplasm
Documents: Manual-BAX Monoclonal Antibody




Product Usage Information

IHC

Human, Mouse, Rat

1: 500-1: 1000

colon cancer, esophageal carcinoma, kidney




Background

Enables Hsp70 protein binding activity and identical protein binding activity. Involved in positive regulation of cysteine-type endopeptidase activity and positive regulation of neuron apoptotic process. Acts upstream of or within several processes, including apoptotic signaling pathway; mitochondrion organization; and regulation of apoptotic process. Located in cytosol; endoplasmic reticulum; and mitochondrial outer membrane. Part of BAX complex. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; genitourinary system; and sensory organ. Human ortholog(s) of this gene implicated in acute lymphoblastic leukemia; ataxia telangiectasia; colon adenocarcinoma; and colorectal cancer. Orthologous to human BAX (BCL2 associated X, apoptosis regulator).




Images

Bax Rabbit mAb

IHC analysis of Bax (GB154122).

Sample: Human colon cancer, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Bax monoclonal antibody

IHC analysis of Bax (GB154122).

Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of Bax (GB154122).

Sample: Mouse kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of Bax (GB154122).

Sample: Mouse liver, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of Bax (GB154122).

Sample: Rat kidney, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of Bax (GB154122).

Sample: Rat liver, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.




Storage

Storage             Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


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