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Anti-Bcl-2 Rabbit pAb (100 μl)
| Reactivity: | M |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Apoptosis regulator Bcl-2 |
Synonyms: | BCL2, Bcl-2, PPP1R50, B-cell CLL/lymphoma 2, apoptosis regulator |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse BCL-2 |
Uniprot ID: | P10417 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus, Endoplasmic reticulum |
Product Usage Information
IHC/IF | Mouse | 1: 500-1: 1000 | B16 cell lung metastatic carcinoma model, spleen |
Background
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Images
| IHC analysis of bcl-2 (GB114830). Sample: Mouse B16 cell lung metastatic carcinoma model (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of bcl-2 (GB114830). Sample: Mouse spleen (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
