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| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Brd4 rabbit polyclonal antibody |
Gene Name: | Bromodomain-containing protein 4 |
Synonyms: | Mitotic chromosome-associated protein, HUNK1bromodomain-containing protein 4, HUNK1,MCAP, Brd4, Mcap |
Immunogen: | Recombinant protein corresponding to Mouse Brd4 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 156 kDa |
Observed MW. | 180 kDa |
Uniprot ID: | O60885, Q9ESU6, D3ZGX8 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, lymph node |
Background
BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms, longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.
Images
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Western blot analysis of brd4 (GB11999) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
