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| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Bub3 rabbit polyclonal antibody |
Gene Name: | Mitotic checkpoint protein BUB3 |
Synonyms: | WD repeat type I transmembrane protein A72.5, Bub3 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Bub3 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Uniprot ID: | O43684, Q9WVA3 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 3000 | brain, breast cancer, lung, lung cancer, pancreas, small intestine, thyroid gland |
Background
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20.
Images
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Immunohistochemistry of paraffin embedded human breast cancer using BuB3 (GB111183) at dilution of 1:1400 (400x lens) |
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Immunohistochemistry of paraffin embedded human lung cancer using BuB3 (GB111183) at dilution of 1:1400 (400x lens) |
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Immunohistochemistry of paraffin embedded human thyroid gland using BuB3 (GB111183) at dilution of 1:1400 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse lung using BuB3 (GB111183) at dilution of 1:1400 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse pancreas using BuB3 (GB111183) at dilution of 1:1400 (400x lens) |
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Immunohistochemistry of paraffin embedded rat brain using BuB3 (GB111183) at dilution of 1:1400 (400x lens) |
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Immunohistochemistry of paraffin embedded rat lung using BuB3 (GB111183) at dilution of 1:1400 (400x lens) |
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Immunofluorescence of paraffin embedded human breast cancer using BuB3 (GB111183) at dilution of 1:1300 (400x lens) |
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Immunofluorescence of paraffin embedded mouse brain using BuB3 (GB111183) at dilution of 1:1300 (400x lens) |
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Immunofluorescence of paraffin embedded rat pancreas using BuB3 (GB111183) at dilution of 1:1300 (400x lens) |
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Immunofluorescence of paraffin embedded rat small intestine using BuB3 (GB111183) at dilution of 1:1300 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
