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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Complement component C6 |
Synonyms: | C6, complement component 6, Complement component C6 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human C6 |
Uniprot ID: | P13671 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Secreted |
Product Usage Information
IHC/IF | Mouse, Rat | 1: 100-1: 300 | liver, spleen, kidney, liver cancer |
Background
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
Images
| IHC analysis of C6 (GB115483). Sample: Mouse liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0)(G1203), 98°C 20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of C6 (GB115483). Sample: Mouse spleen (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0)(G1203), 98°C 20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of C6 (GB115483). Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0)(G1203), 98°C 20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of C6 (GB115483). Sample: Rat liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0)(G1203), 98°C 20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of C6 (GB115483). Sample: Human liver cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0)(G1203), 98°C 20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
