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| Reactivity: | H & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | T-cell surface glycoprotein CD3 epsilon chain |
Synonyms: | CD3E, IMD18, T3E, TCRE, CD3e molecule |
Immunogen: | Recombinant protein corresponding to Human CD3 |
Uniprot ID: | P07766 |
Isotype: | IgG2b,κ |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 20-25 kDa / 23 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | Jurkat, Molt-4 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1500 | tonsil, spleen, mouse 4T1 breast tumor model |
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Images
| Western blot analysis of CD3 (GB12014) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using CD3 (GB12014) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse spleen using CD3 (GB12014) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse mouse 4T1 breast tumor model using CD3 (GB12014) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat spleen using CD3 (GB12014) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
