Your shopping cart is empty!
Recombinant Anti-CD3 Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | T-cell surface glycoprotein CD3 epsilon chain |
Synonyms: | CD3E, IMD18, T3E, TCRE, CD3e molecule |
Immunogen: | Recombinant protein corresponding to Human CD3 |
Uniprot ID: | P07766 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 20-25 kDa / 23 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 2000 | thymus |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | tonsil, spleen |
Background
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z.All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways.
Images
| WB analysis of CD3(GB15014). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of CD3(GB15014). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of CD3(GB15014). Sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of CD3(GB15014). Sample: Rat spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
