Recombinant Anti-CD74 Mouse mAb (100 μl)

HLA class II histocompatibility antigen gamma chain

Synonyms:

CD74, DHLAG, HLADG, Ia GAMMA, Ii, p33, CLIP, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain

Immunogen:

Recombinant human CD74 protein expressed in HEK293 cells

Uniprot ID:

P04233

Isotype:

IgG1

Purity:

Affinity purification

Subcellular location:

Cell membrane, Endosome, Lysosome, Golgi apparatus, Endoplasmic reticulum

Predicted MW. / Observed MW.

34 kDa / 34 kDa

WB

Human

1: 500-1: 1000

Daudi, Raji

IHC/IF

Human

1: 500-1: 2000

colon, tonsil

WB analysis of CD74(GB151179).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h.

IHC analysis of CD74(GB151179).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

IHC analysis of CD74(GB151179).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.