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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | CDC25 rabbit polyclonal antibody |
Gene Name: | Ras-specific guanine nucleotide-releasing factor 1 |
Synonyms: | RASGRF1, CDC25, CDC25L, GNRP, GRF1, GRF55, H-GRF55, PP13187, Ras-GRF1, Ras protein specific guanine nucleotide releasing factor 1 |
Immunogen: | Recombinant protein corresponding to Mouse CDC25 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm |
Uniprot ID: | Q13972 , P28818 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Mouse, Rat | 1: 1000-1: 4000 | brain |
Background
The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit.
Images
 | Immunohistochemistry of paraffin embedded mouse brain using rasgrf1 (GB11856) at dilution of 1:4000 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat brain using rasgrf1 (GB11856) at dilution of 1:4000 (400x lens) |
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Immunofluorescence of paraffin embedded mouse brain using rasgrf1 (GB11856) at dilution of 1:2000 (400x lens) |
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Immunofluorescence of paraffin embedded rat brain using rasgrf1 (GB11856) at dilution of 1:2000 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
