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Recombinant Anti-CDX2 Mouse mAb (100 μl)
| Reactivity: | H & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Homeobox protein CDX-2 |
Synonyms: | caudal type homeobox 2, Caudal type homeobox protein 2, CDX 3, CDX2, CDX-2, CDX3, Homeobox protein CDX 2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human CDX2 |
Uniprot ID: | Q99626 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 34 kDa / 38 kDa |
Product Usage Information
WB | Human | 1: 500-1: 2000 | Caco-2, HeLa, MCF7 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2000 | colon cancer, colon |
Background
Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity).
Images
| WB analysis of CDX2(GB151501). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of CDX2(GB151501). Sample: Human colon cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IHC analysis of CDX2(GB151501). Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IHC analysis of CDX2(GB151501). Sample: Mouse colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IHC analysis of CDX2(GB151501). Sample: Rat colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
