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| Key Features: | • Superior performance: Bright, single-peak staining enables visualization of multiple generations. • Long-term signal stability: Well-retained in cells for several days post stain. • Non-toxic: Staining does not adversely effect cell health • Simple, robust staining protocol |
| Description: | LiFluor™ CFSE Cell Proliferation Kit provides convenient single-use vials for cell labeling. CFSE [5-(and 6)-carboxy fluorescein diacetate, succinimidyl ester] is a fluorescent tracer that diffuses passively into cells and covalently labels intracellular proteins, resulting in long term cell labeling. It is non-fluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases in viable cells. After hydrolysis, the succinimidyl ester group reacts with intracellular amines forming fluorescent conjugates that are retained in the cells. The excess unconjugated CFSE diffuses passively back to the extracelluar medium and can be rinsed away. After labeling, cells can be fixed with formaldehyde or gluteraldehyde. The kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. |
| Specifications: | 1. Platform: Flow Cytometer 2. Detection Method: Fluorescent 3. Ex/Em: 490/517 nm |
| Applications: | Cell generation analysis by flow cytometry. |
| Component: | 1. 10× CFSE: 500 µg 2. Anhydrous DMSO: 1 ml |
| Storage: | At -20°C and Protect from light |
| Documents | Manual |
Cell generation analysis with CFSE Cell Proliferation Kit. Jurkat cells (~1×106 cells/ml) were stained with CFSE dye (1 µM) on Day 0. The cells were cultured for 7 days. Fluorescence intensity was measured with FACS flow cytometer.
