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Anti-COX5B Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | COX5B rabbit polyclonal antibody |
Gene Name: | Cytochrome c oxidase subunit 5B, mitochondrial |
Synonyms: | Cytochrome c oxidase polypeptide Vb, Cox5b, COXIV, cytochrome c oxidase polypeptide IV, Cytochrome c oxidase polypeptide VIa |
Immunogen: | Recombinant protein corresponding to Mouse COX5B |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. | 14 kDa |
Observed MW. | 14 kDa |
Uniprot ID: | P10606, P19536, P12075 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | glioma, heart, liver |
IHC/IF | Human, Rat | 1: 1000-1: 2000 | melanoma, liver |
Background
COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.
Images
| Western blot analysis of COX5B (GB111551) at dilution of 1: 500 |
| Immunofluorescence of paraffin embedded human melanoma using CA14 (GB111551) at dilution of 1: 1000 (400x lens) |
| Immunofluorescence of paraffin embedded rat liver using CA14 (GB111551) at dilution of 1: 1000 (400x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using COX5B (GB111551) at dilution of 1: 1000 (200x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HepG2 cells using COX5B (GB111551) at dilution of 1: 1000 (200x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed MCF-7 cells using COX5B (GB111551) at dilution of 1: 1000 (200x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
