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Anti-Calreticulin Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Calreticulin rabbit polyclonal antibody |
Gene Name: | Calreticulin |
Synonyms: | CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, Calr, Autoantigen RO, CRTC, Sicca syndrome antigen A, SSA, CRT, RO, cC1qR |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Calreticulin |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Endoplasmic reticulum, Lysosome, Secreted |
Predicted MW. | 48 kDa |
Observed MW. | 55 kDa |
Uniprot ID: | P27797, P14211, P18418 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, kidney |
IHC/IF | Mouse | 1: 250-1: 500 | stomach, substantia nigra |
Background
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species.
Images
| Western blot analysis of CALR (GB112134) at dilution of 1: 500 |
| Immunofluorescence of paraffin embedded mouse stomach using CALR (GB112134) at dilution of 1: 250 (350x lens) |
| Immunofluorescence of paraffin embedded mouse substantia nigra using CALR (GB112134) at dilution of 1: 250 (200x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
