Your shopping cart is empty!
Anti-Caspase-3 Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Caspase-3 |
Synonyms: | Casp3, CASP3, CPP32, CPP32B, SCA-1, Caspase 3 |
Immunogen: | Recombinant protein corresponding to Human Caspase-3 |
Uniprot ID: | P42574 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 32 kDa / 35/17 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | Jurkat, THP-1, 293T |
IHC/IF | Human | 1: 700-1: 2100 | tonsil, colon, T-cell lymphoma |
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly polymerase at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
Images
| Western blot analysis of Caspase-3 (GB115600) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human colon using Caspase-3 (GB115600) at dilution of 1:700 |
| Immunohistochemistry analysis of paraffin embedded human T-cell lymphoma using Caspase-3 (GB115600) at dilution of 1:700 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using Caspase-3 (GB115600) at dilution of 1:700 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
