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| Key Features: | • Simple: Simultaneous detection of apoptotic, necrotic, and total cells in a single assay • Fast: A single 30 min staining step • Sensitive: Brighter fluorescence signal |
| Description: | Apoptotic, Necrotic & Healthy Cells Detection Kit provides a convenient assay to detect apoptotic, necrotic, and total cells within the same cell population by flow cytometry or fluorescence microscopy. This kit contains three-color reagent: LiFluor™ 488 annexin V used to stain apoptotic cells by binding to phosphatidylserine (PS) exposed on the cell surface; propidium iodide (PI) used to stain necrotic cells with comprised membrane integrity; and Hoechst 33324 used to stain all cell nuclei. After staining a cell population, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and all cells show blue fluorescence. |
| Specifications: | 1. Platform: Fluorescence Cytometer, Fluorescence Microscopy 2. Detection Method: Fluorescent 3. Ex/Em: PI: 535/617 nm; LiFluor™ 488: 495/520 nm; Hoechst 33324: 350/460 nm |
| Applications: | Cell apoptosis analysis |
| Component: | 1. LiFluor 488 Annexin V: 250 µl 2. PI: 250 µl 3. 5Hoechst 33342: 250 µl 4. 5× Annexin-binding buffer: 25 ml |
| Storage: | At 4°C and protect from light |
| Download: | Manual |