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The LiFluor™ 555 EdU Imaging Kit emerges as a superior choice over the traditional BrdU cell proliferation assay, specifically tailored for fluorescence microscopy. Within this assay, the modified thymidine analogue EdU is seamlessly integrated into newly synthesized DNA strands and then labeled with a vivid, photostable LiFluor™ dye through a rapid and highly-specific click reaction. This method of fluorescently marking proliferating cells is not only precise but also compatible with antibody techniques, owing to the gentle nature of the click protocol.
Key Features
• Simple: Labeling is complete in two steps.
• Efficient: No denaturation steps or harsh treatment required.
• Content-rich results: Better preservation of cell morphology, antigen structure, and DNA integrity.
• Consistent: Not dependent on variable antibody lots for detection.
Specifications
1. Platform: Fluorescence Microscope
2. Detection Method: Fluorescent
3. Ex/Em: 555/565 nm
Applications
Cell proliferation analysis in vitro and in vivo.
Components
1. EdU: 2×1 ml
2. LiFluor 555 azide: 100 µl
3. CEdU reaction buffer: 50 ml
4. CuSO4: 1 ml
5. EdU buffer additive: 200 mg
6. Hoechst 33342: 70 µl
Storage
Store at -20°C and protect from light.
Case Study
Detection of cell proliferation in cell. A549 cells were treated with 10 µM EdU for 2 hr, then detected with LiFluor™ 555 azide (red), cells were counterstained with DAPI (blue).
