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| Key Features: | • Simple workflow: add, incubate, and read format, no wash needed. • Flexibility: suitable for flow cytometry, fluorescence microscope, and plate reader. • Fast: results in as little as 30 minutes. |
| Description: | Mitochondrial Membrane Potential Detection Kit provides a quick and reliable method to detect the mitochondrial membrane potential changes in apoptotic cells using flow cytometry. The loss of mitochondrial membrane potential is a hallmark for apoptosis. The JC-1 Assay Kit uses a unique cationic dye (5,5’,6,6’- tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide) to signal the loss of mitochondrial membrane potential. JC-1 exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (529 nm) to red (590 nm). Consequently, mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. In healthy cells, the JC-1 accumulates in the mitochondrial to form J-aggregates, which become red fluorescence. In apoptotic cells, the JC-1 cannot accumulate within the mitochondria and remains in the cytoplasm in monomeric form to show green fluorescence. |
| Applications: | Mitochondrial Membrane Potential Assay |
| Component: | 1. JC-1: 5×100 µl, 100× 2. CCCP: 125 µl, 25 mM in DMSO |
| Storage: | At -20°C and Avoid repeated freeze-thaw cycles |
| Download: | Manual |
Detection of cell apoptosis with JC-1 Mitochondrial Membrane Potential Detection Kit. Jurkat cells treated with 10 μM camptothecin for 4 hr (right panel) or untreated (left panel). Cells were stained with JC-1 reagent, then analyzed by flow cytometry.