Lentivirus Transduction Enhancer II is a nontoxic novel chemical reagent that increases lentiviraltransduction efficiency by modulating membrane permeability. Normally, incubation of lentivirusparticles in cells in the presence of polybrene is generally efficient in transducing many cell types.However, due to polycation property, polybrene is toxic for some sensitive cells. LentivirusTransduction Enhancer II is a neutral and nontoxic transduction enhancer, which significantlyincreases lentivirus transducing efficiency while maintaining cell membrane integrity.
Lentivirus Transduction Enhancer II is most effective when added to cell culture media at the time oftransduction. Recommended working concentration ranges from 1:100 to 1:50. However, the workingconcentration is highly cell line-dependent. Lower or higher dilution ratio may be required to optimizethe effect.
Component
1. Lentivirus Transduction Enhancer II: 1 ml
Storage
Store at -20°C.
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