LiFectRNA™ Transfection Reagent (1 ml)

LiFectRNA™ Transfection Reagent is a novel biodegradable non-liposomal siRNA/miRNA & DNA delivery tool. With our proprietary pH Dependent Conformational Change (PDCC) technology, the biodegradable transfection reagent was formulated by addition of pre-screened hydrophobic groups to its side chain, making LiFectRNA™ Reagent a versatile and most powerful gene delivery tool in the market. LiFectRNA™ Reagent have been validated to effectively and reproducibly transfect single siRNA/miRNA or co-transfect DNA/siRNA or DNA/miRNA to variety of mammalian cells.
 

Key Features

• Excellent silencing at low concentrations with only 1.0 nM siRNA/miRNA mimics.

• Minimizing non-specific and off target effects in the cell.

• One tube reaction.

• Best for broad mammalian cells.

• Very low cytotoxicity.
 

Applications

1. Single siRNA transfection or DNA/siRNA co-transfection to variety of mammalian cells.

2. Single miRNA, miRNA mimics or inhibitors transfection or DNA/miRNA or DNA/miRNA mimics co-transfection to variety of mammalian cells.
 

Components

1. LiFectRNA™ Reagent: 1.0 ml
2. LiFectRNA™ Transfection Buffer (5×): 8.0 ml

Storage

Store at 4 °C for LiFectRNA™ Reagent and RT for LiFectRNA™ Transfection Buffer (5×).
 

Case Study

Excellent silencing of endogenously expressed KIF11 (also known as Eg5) in HEK293 cells with 0.5 µl of LiFectRNA™ reagent and 5.0 pmol Eg5 siRNA per well of 24-well plate. KIF11 (also known as Eg5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LiFectRNA™ reagent effectively delivers Eg5 siRNA (final 10 nM, right panel) to HEK293 cells at only 0.5 µl per well of 24-well plate vs. negative control (final 10 nM. left panel). Compared with negative control (left panel), phenotype of "rounded-up" 293 cells were visualized 24 hours post transfection (right panel) with a Nikon microscope.

LiFectRNA™ Transfection Reagent knocked down endogenous lamin A/C gene expression in Hela cells. A siRNA targeting lamin A/C gene (right panel) and a sham siRNA (left panel) were introduced into Hela cells (final 1.0 nM) by LiFectRNA™ Transfection Reagent. Lamin A/C gene silencing was monitored 24h post transfection by immunofluorescence. Lamin A/C was probed with a mouse monoclonal lamin A/C specific antibody followed by addition of FITC-conjugated anti-mouse antibodies. Quantitative analysis showed that lamin siRNA at 1.0 nM delivered by LiFectRNA™ Transfection Reagent knocked down 94% endogenously expressed lamin A/C in Hela cells.