As a cationic polymer, polybrene increases the efficiency of infection in some cells by neutralizing the charge repulsion between virions and the cell surface. Polybrene is most effective when added to cell culture media at the time of transduction. Recommended working concentration is 1:100. However, the working concentration is highly cell line-dependent. Lower or higher dilution ratio may be required to optimize the effect.
Component
1. Polybrene: 1 ml, 0.8 mg/mL
Storage
Store at -20°C.
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