5× SDS-PAGE Loading Buffer (25 ml)

SDS-PAGE loading buffer, a crucial component for preparing protein samples for electrophoresis, typically contains SDS, glycerol, a reducing agent (like DTT or BME), Tris-HCl, and a tracking dye (like bromophenol blue). The SDS-PAGE Sample Loading Buffer is a 5× solution of 250 mM Tris·HCl, pH 6.8, 10% SDS, 30% (v/v) Glycerol, 10 mM DTT, 0.05% (w/v) Bromophenol Blue for use in SDS-polyacrylamide gel electrophoresis of proteins.
 

Key Features

• Universal Compatibility: Works with Laemmli and Tris-glycine systems.

• Ready-to-Use: Pre-optimized 5× SDS-PAGE Sample Loading Buffer formulation.

• Consistency: Batch-tested for reproducible western blot results.
 

Applications

Protein gel electrophoresis
 

Component

1. 5× SDS-PAGE Loading Buffer: 25 ml
 

Storage

Store at -20°C.