Serum-Free Medium for Lymphocyte (1000 ml)

The Lymphocyte Superum-free Medium is an advanced formulation developed as an enhancement to the L100 serum-free medium, specifically designed for the in vitro cultivation of human lymphocytes. This medium is meticulously crafted with a chemically defined composition, ensuring that all ingredients are sourced from cell culture-grade materials. It is devoid of heterogeneous animal components, serum, or any protein additives beyond albumin, transferrin, and insulin, thereby effectively mitigating the impact of serum quality variations and the presence of exogenous components on experimental outcomes. This medium facilitates the high-density cultivation of various immunocytes, including human lymphocytes, cytokine-induced killer cells, natural killer cells, and dendritic cells.
 

Applications

The Serum-Free Medium for Lymphocyte supports the high-density suspension culture of lymphocytes. It supports the suspension culture of lymphocytes with the density of 5×105 ~5×106 cells/mL in a static culture container. To ensure sufficient gas exchange of the culture system, the recommended depth of the medium is 1~1.5 cm. The medium also supports the high-density culture of lymphocytes in a bioreactor, and the optimization of the culture procedure should be determined by researchers based on experience. The serum-free medium for lymphocyte supports the serum-free culture of immunocytes, and the addition of autologous plasma, human AB serum, FBS or serum substitutes can significantly improve the cell viability and increase the cell proliferation rate. The cell culture supplemental mix is recommended to obtain the optimal culture result.
 

Component

1. Serum-Free Medium for Lymphocyte (1000 ml): 1000 ml
 

Storage

Store at 4 °C.