Mycoplasma PCR Detection Kit (50 T)

Rapid Mycoplasma PCR in Cell Cultures

Mycoplasma contamination remains one of the most critical challenges in laboratory cell culture maintenance, known to compromise experimental validity through altered cell growth rates, chromosomal abnormalities, and disrupted metabolic processes. Our Mycoplasma PCR Detection Kit provides researchers with a cutting-edge solution for identifying these persistent contaminants with unmatched precision.

The kit contains an optimized PCR master mix and a positive control. The primers in the PCR master mix are highly specific to the conserved rDNA region in the mycoplasma genomes and can detect all well-known mycoplasma genera, including the commonly encountered ones in cell cultures, such as M. orale, M. hyorhinis, M. laidlawii, M. salivarium, M. arginini, M. fermentans, M. hominis, and M. pneumoniae. Mycoplasma positive samples can be easily recognized by a distinctive PCR product ranging in size from 400 to 600 bp.
 

Key Features of Our Mycoplasma Detection Kit

• Ultra-Sensitive PCR Technology: Specifically detects mycoplasma DNA in cell culture supernatant through optimized primer design.

• Time-Efficient Protocol: Delivers results in <2 hours - 3x faster than conventional methods.

• Clear Visual Confirmation: Simplified gel electrophoresis analysis with included positive controls.

• Comprehensive Detection: Identifies all common mycoplasma species affecting cell cultures.
 

Application

Mycoplasma Detection

Components

1. 2× PCR Master Mix: 640 µl
2. Positive Control: 45 µl
 

Storage

Store at -20°C and Avoid repeated freeze-thaw cycles
 

Case Study

Mycoplasma detection PCR in cell cultures