The CCK-8 Cell Counting Kit facilitates sensitive colorimetric assays for assessing cell viability in both cell proliferation and cytotoxicity studies. This kit utilizes the highly water-soluble tetrazolium salt, WST-8, which is metabolized by cellular dehydrogenase enzymes to produce a yellow-colored formazan dye that remains soluble within the tissue culture medium. The concentration of formazan dye, a byproduct of dehydrogenase activity within cells, is directly proportional to the number of viable cells present. The CCK-8 kit boasts superior detection sensitivity compared to other tetrazolium salts, including MTT, XTT, MTS, and WST-1, making it an excellent choice for accurate cell viability measurements.
Key Features
• Quick and Simple Protocol
• High sensitivity
Specifications
1. Platform: Microplate Reader
2. Detection Method: Colorimetric
3. OD: 450 nm
Applications
1. Cell proliferation analysis
2. Cell viability assays, including cytotoxicity
3. Cytokine assays
Component
1. CCK-8 Solution: 5 ml
Storage
Store at 4°C and protect from light.
Case Study