LiFluor™ Green Cell Viability Imaging Kit is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of Hoechst 33342 Live Cell Stain reagent and Green 488 Dead Cell Stain reagent to 1 ml of cell growth media, then determine viability by counting total vs dead cells. Hoechst 33342 reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. Green 488 Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC (green) filter set. This kit is appropriate for fluorescence microscopy and flow cytometry.
Specifications
1. Compatible Cells: Eukaryotic Cells, Mammalian Cells
2. Detection Method: Fluorescent
3. Ex/Em: Hoechst 33342 reagent 350/461 nm; Green 488 Dead reagent 500/530 nm
Applications
Cell viability assay
Components
1. Hoechst 33342 Live Cell Stain: 10 ml
2. Green 488 Dead Cell Stain: 10 ml
Storage
Store at 4°C and protect from light.