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Anti-Clathrin light chain Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Clathrin light chain rabbit polyclonal antibody |
Gene Name: | Clathrin light chain B |
Synonyms: | Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB |
Immunogen: | Recombinant protein corresponding to Mouse Clathrin light chain |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Membrane |
Predicted MW. | 25 kDa |
Observed MW. | 35 kDa |
Uniprot ID: | Q6IRU5 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 3000-1: 6000 | brain |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | brain, testis, liver cancer |
Background
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)
Images
 | Western blot analysis of CLTB (GB114034) at dilution of 1: 5000 |
 | Immunohistochemistry of paraffin embedded human hepatobiliary cancer using CLTB (GB114034) at dilution of 1: 3000 |
 | Immunohistochemistry of paraffin embedded human liver cancer using CLTB (GB114034) at dilution of 1: 3000 |
 | Immunohistochemistry of paraffin embedded mouse brain using CLTB (GB114034) at dilution of 1: 700 |
 | Immunohistochemistry of paraffin embedded rat brain using CLTB (GB114034) at dilution of 1: 700 |
 | Immunohistochemistry of paraffin embedded mouse testis using CLTB (GB114034) at dilution of 1: 1500 |
 | Immunohistochemistry of paraffin embedded rat testis using CLTB (GB114034) at dilution of 1: 1500 |
 | Immunofluorescence of paraffin embedded human hepatobiliary cancer using CLTB (GB114034) at dilution of 1: 1000 |
 | Immunofluorescence of paraffin embedded human liver cancer using CLTB (GB114034) at dilution of 1: 1000 |
 | Immunofluorescence of paraffin embedded mouse brain using CLTB (GB114034) at dilution of 1: 1000 |
 | Immunofluorescence of paraffin embedded mouse testis using CLTB (GB114034) at dilution of 1: 1000 |
 | Immunofluorescence of paraffin embedded rat testis using CLTB (GB114034) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
