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| Reactivity: | Human, Mouse, Rat, Bovine, Chicken, Dog |
| Applications: | WB, IH |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Rabbit anti-Collagen-pan Antibody |
| Synonyms: | Collagen alpha-1(I) chain; Alpha-1 type I collagen; Collagen alpha-1(II) chain; Alpha-1 type II collagen; Collagen alpha-1(III) chain |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen-pan. The exact sequence is proprietary. |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:100 |
| Gene ID (Human): | 1277; 1280; 1281; 1289 |
| Gene ID (Mouse): | 12842; 12824; 12825; 12831 |
| Gene ID (Rat): | 29393; 25412; 84032; 85490 |
| SwissProt (Human): | P02452; P02458; P02461; P20908 |
| SwissProt (Mouse): | P11087; P28481; P08121; O88207 |
| SwissProt (Rat): | P02454; P05539; P13941; Q9JI03 |
| Purification Method: | The antibody was purified by immunogen affinity chromatography. |
| Concentration: | 1mg/mL |
| Buffer: | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Documents: | Manual-Collagen-pan Antibody |
Background
The Collagen-pan antibody was generated using COL1A1, COL2A1, COL3A1, and COL5A1 as the antigen and it reacts with Human, Mouse, Rat, Bovine, Chicken, Dog.
Images
 | Western blot analysis of Collagen-pan expression in NIH3T3 (A) whole cell lysates. |
 | Immunohistochemical analysis of Collagen-pan staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
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