Cy3-conjugated Goat anti-Rabbit IgG (H+L) (200 μl)

Cy3-conjugated Goat anti-Rabbit IgG (H+L) (200 μl)

Brand: Abclonal
Cat. #: AS007
Availability: In Stock
$65.00
-+
Applications: IF/IC, FC    
Host Species: Goat    
Isotype: Cy3 conjugated IgG    
Conjugation: Cy3. Ex:548nm. Em:562nm.    
Immunogen: Rabbit IgG
Dilution: IF/IC 1:100-1:800; FC 1:100-1:800
Purification Method: Affinity purification
Concentration: 1.02 mg/mL
Buffer: PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual




Images

Cy3-conjugated Goat anti-Rabbit IgG (H+L)Confocal imaging of U-2 OS cells using DNA topoisomerase I (TOP1) Rabbit mAb(A12409,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:100)(Red),ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L)(AS076,dilution 1:200) (Green)
Flow cytometry: 1X10^6 K-562 cells (negative control,left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb (5 μl/Test,orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). Cy3 Goat Anti-Rabbit IgG (H+L)(AS007, 1:800) was used as a secondary antibody.

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