Cy5-X-UTP is supplied as a 1 mM solution in TE buffer. Cy5-X-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases, as well as other RNA modifying enzymes. The X linker between Cy5 and UTP can improve the incorporation efficiency.
Key Features
• Bright signals with low background.
• Wide variety of fluorophores.
• Great for many applications, like Fluorescence in situ Hybridization, TUNEL and more.
Specifications
1. Excitation/Emission (nm): 650/665 nm
2. Concentration: 1 mM solution in TE buffer
3. Molecular Formula: C50H63N6Na4O23P3S2
4. Molecular Weight: 1365.07
Applications
Indirect non-radioactive enzymatic labeling of RNA during in vitro transcription.
Component
1. Cy5-X-UTP: 25 μl
Storage
Store at -20°C and protect from light.