Cy5-X-dUTP is supplied as a 1 mM solution in TE buffer. Cy5-X-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The X linker between Cy5 and dUTP can improve the incorporation efficiency.
Key Features
• Bright signals with low background.
• Wide variety of fluorophores.
• Great for many applications, like Fluorescence in situ Hybridization, TUNEL and more.
Specifications
1. Excitation/Emission (nm): 650/665 nm
2. Concentration: 1 mM solution in TE buffer
3. Molecular Formula: C50H63N6Na4O22P3S2
4. Molecular Weight: 1349.07
Applications
Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.
Component
1. Cy5-X-dUTP: 25 μl
Storage
Store at -20°C and protect from light.