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Anti-Cyclin B1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | G2/mitotic-specific cyclin-B1 |
Synonyms: | Ccn-2, Ccnb1-rs13, Cycb, Cycb1, Ccnb1, CCNB |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Cyclin B1 |
Uniprot ID: | P24860 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton, Nucleus |
Predicted MW. / Observed MW. | 48 kDa / 55 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis, colon, spleen |
IHC/IF | Human, Mouse, Rat | 1: 400-1: 3000 | colon cancer, skin, colon, lung cancer, cervical cancer, testis |
Background
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Images
| Western blot analysis of CYCLINB1 (GB112098) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human skin using Cyclin B1 (GB112098) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded human colon cancer using Cyclin B1 (GB112098) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded human skin using Cyclin B1 (GB112098) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded mouse colon using Cyclin B1 (GB112098) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat colon using Cyclin B1 (GB112098) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human lung cancer using Cyclin B1 (GB112098) at dilution of 1: 800 |
| Immunofluorescent analysis of paraformaldehyde-fixed human cervical cancer using Cyclin B1 (GB112098) at dilution of 1: 800 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using Cyclin B1 (GB112098) at dilution of 1: 800 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat testis using Cyclin B1 (GB112098) at dilution of 1: 800 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat colon using Cyclin B1 (GB112098) at dilution of 1: 800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
