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Anti-Cytokeratin 15 Mouse mAb (100 μl)
| Reactivity: | H & H,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Keratin, type I cytoskeletal 15 |
Synonyms: | Cytokeratin-15, CK-15, Keratin-15, K15, Krt1-15, KRTL15, Keratin-15, K1CO, KRTB, Type I keratin Ka15 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to human Cytokeratin15 |
Uniprot ID: | P19012 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoskeleton |
Predicted MW. / Observed MW. | 49 kDa / 49 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human | 1: 500-1: 1000 | A431 |
IHC/IF | Human, Rat | 1: 1000-1: 2000 | esophagus, cervical cancer, tonsil |
Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.
Images
| Western blot analysis of CK15 (GB121648) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human esophagus using CK15 (GB121648) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded human cervical cancer using CK15 (GB121648) at dilution of 1: 2000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human esophagus using CK15 (GB121648) at dilution of 1: 2000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human tonsil using CK15 (GB121648) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
