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Recombinant Anti-Cytokeratin 15 Mouse mAb (100 μl)
| Reactivity: | H & H,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Keratin, type I cytoskeletal 15 |
Synonyms: | Cytokeratin-15, CK-15, Keratin-15, K15, Krt1-15, KRTL15, Keratin-15, K1CO, KRTB, Type I keratin Ka15 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Cytokeratin15 |
Uniprot ID: | P19012 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Cytoskeleton, cytosol, nucleus |
Predicted MW. / Observed MW. | 49 kDa / 49 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human | 1:500-1:1500 | A-431 |
IHC/IF | Human, Rat | 1: 250-1: 1000 | esophageal cancer, skin |
Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.
Images
| WB analysis of Cytokeratin 15(GB151648). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of Cytokeratin 15(GB151648). Sample: Human esophageal cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Cytokeratin 15(GB151648). Sample: Rat skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
