Recombinant Anti-Cytokeratin 6 Rabbit mAb (100 μl)

Recombinant Anti-Cytokeratin 6 Rabbit mAb (100 μl)

Brand: ServiceBio
Cat. #: GB151247-100
Availability: In Stock
$259.00
-+
Reactivity: H,M,R    
Applications: IHC/IF    
Host Species: Rabbit    
Clonality: Monoclonal    
Gene Name:

Keratin, type II cytoskeletal 6A

Synonyms:

CK-6A, CK 6D, CK6A, CK6C, CK6D, Cytokeratin 6A, Cytokeratin 6D, Hom s 5, K6A, K6C, K6D, keratin 6A, KRT conserve, KRT6, KRT6A, KRT6C, KRT6D, Type II keratin Kb6

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human CK6

Uniprot ID:

P02538

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Cytoplasm




Product Usage Information

IHC/IF

Human, Mouse, Rat

1: 500-1: 1000

Skin, esophageal carcinoma




Background

Keratin 6A is a member of the keratin gene family which is one of the 27 different type II keratinsis. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin(KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia.




Images

01 GB151247_Cytokeratin 6_ZSR22059_ZSR22059231208_d500_M skin_20.0x_IHC_WM.png

IHC analysis of Cytokeratin 6(GB151247).
Sample: Mouse skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02 GB151247_Cytokeratin 6_ZSR22059_ZSR22059231208_d500_R skin_20.0x_IHC_WM.png

IHC analysis of Cytokeratin 6(GB151247).
Sample: Rat skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03 GB151247_Cytokeratin 6_ZSR22059_ZSR22059231208_d700_H esophageal carcinoma_20.0x_IHC_WM.png

IHC analysis of Cytokeratin 6(GB151247).
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 700, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.




Storage

Storage             Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


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