Reactivity: | H,M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | DAGLB rabbit polyclonal |
Gene Name: | Diacylglycerol lipase-beta |
Synonyms: | DAGL-beta, DAGLbeta, DGL-beta, PUFA-specific triacylglycerol lipase, Sn1-specific diacylglycerol lipase beta, Daglb, KCCR13L |
Immunogen: | Recombinant protein corresponding to Mouse DAGLB |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 74 kDa |
Observed MW. | 74 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | Brain, testis |
Background
DAGL beta uses calcium as a cofactor to catalyze the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), a reaction that is required for axonal growth and for retrograde synaptic signaling at mature synapses. DAGL beta functions at an optimal pH of 7 and its activity is inhibited by p-hydroxy-mercuri-benzoate and HgCl2, but not PMSF. There are three isoforms of DAGL beta that are produced as a result of alternative splicing events.
Images
Western blot analysis of DAGLB (GB113076) at dilution of 1: 1000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |